Product Description
Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Recombinant human CKMT1A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Biovision | P1578 | Creatine Kinase MT, Human Recombinant DataSheet
Biomolecule/Target :
Synonyms: Creatine kinase U-type, mitochondrial, Acidic-type mitochondrial creatine kinase, Mia-CK, Ubiquitous mitochondrial creatine kinase, U-MtCK, CKMT1A, CKMT
Alternates names: Creatine kinase U-type, mitochondrial, Acidic-type mitochondrial creatine kinase, Mia-CK, Ubiquitous mitochondrial creatine kinase, U-MtCK, CKMT1A, CKMT
Taglines: Plays a central role in energy transduction
NCBI Gene ID #: 1159
NCBI Gene Symbol: CKMT1A, CKMT1B
Gene Source: Human
Accession #: P12532
Recombinant: TRUE
Source: E. coli
Purity by SDS-PAGE: >95%
Assay: SDS - PAGE
Purity:
Assay #2:
Endotoxin Level:
Activity (Specifications/test method): > 50 unit/mg
Biological activity:
Results:
Binding Capacity:
Unit Definition: One unit is defined as the amount of enzyme that converts 1.0 umole of phosphate from phosphocreatine to ADP per minute at pH 7.5 at 37C.
Molecular Weight: 45 kDa (403aa) (N-terminus His-Tag)
Concentration:
Appearance: Liquid
Physical form description: In 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 10% glycerol
Reconstitution Instructions:
Amino acid sequence: aa 40-417
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans