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Product Description
Erythropoietin Receptor is a member of the cytokine receptor family. It mediates erythropoietin-induced erythroblast proliferation and differentiation, activation of Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor plays a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis.
Biovision | P1638 | Human CellExp™ Erythropoietin Receptor DataSheet
Biomolecule/Target :
Synonyms: EPOR, EPO-R
Alternates names: EPOR, EPO-R
Taglines: Mediates erythropoietin-induced erythroblast proliferation and differentiation
NCBI Gene ID #: 2057
NCBI Gene Symbol:
Gene Source: Human
Accession #: P19235, NP_000112.1
Recombinant: 1
Source: HEK293 cells
Purity by SDS-PAGE: ≥ 95% by SDS-PAGE
Assay:
Purity:
Assay #2:
Endotoxin Level: < 0.1 EU/ug of the protein as determined by LAL method
Activity (Specifications/test method):
Biological activity: Measured by its ability to inhibit Epo-dependent proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 60-300 ng/ml in the presence of 0.2 U/ml of Recombinant Human EPO Receptor/EPOR.
Results: Measured by its ability to inhibit Epo-dependent proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 60-300 ng/ml in the presence of 0.2 U/ml of Recombinant Human EPO Receptor/EPOR.
Binding Capacity:
Unit Definition:
Molecular Weight: The protein migrates as a band at ~55-60 kDa band in SDS-PAGE.
Concentration:
Appearance: Lyophilized
Physical form description:
Reconstitution Instructions: Reconstitute to a concentration of 0.1-0.5 mg/ml using sterile distilled water
Amino acid sequence: The target protein is expressed with the sequence (Ala25-Pro250) of human Erythropoietin Receptor protein fused to Fc and 6×His tag at the C-terminus.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans
