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Product Description
PON1, also known as A esterase1, is a member of the paraoxonase family. It is an enzyme that hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. It is also a major anti-atherosclerotic component of high-density lipoprotein (HDL). This protein is activated by PPAR-gamma, which increases synthesis and release of paraoxonase 1 enzyme from the liver, reducing atherosclerosis. PON1 shows a variety of atheroprotective properties by metabolizing inflammatory lipid peroxides. It has evolved to be a highly promiscuous enzyme capable of hydrolysing a wide variety of substrates such as lactones, cyclic carbonates, organophosphorus pesticides and nerve gases. Recombinant human PON1, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Biovision | P1556 | Human CellExp™ PON1, Human Recombinant DataSheet
Biomolecule/Target :
Synonyms: Aromatic esterase 1, A-esterase 1, K-45, Serum aryldialkylphosphatase 1, paraoxonase 1, ESA, MVCD5, PON
Alternates names: Aromatic esterase 1, A-esterase 1, K-45, Serum aryldialkylphosphatase 1, paraoxonase 1, ESA, MVCD5, PON
Taglines: Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides
NCBI Gene ID #: P27169
NCBI Gene Symbol: PON1
Gene Source: Human
Accession #: P27169
Recombinant: FALSE
Source: HEK 293 cells
Purity by SDS-PAGE: >90%
Assay: SDS - PAGE
Purity:
Assay #2:
Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)
Activity (Specifications/test method): Specific activity is > 2,500 pmol/min/ug
Biological activity:
Results:
Binding Capacity:
Unit Definition: One unit is defined as the amount of enzyme that hydrolyze 1pmole of pnitrophenyl acetate to p-nitrophenol per minute at pH7.5 at 37C.
Molecular Weight: 39.0 kDa (His-tag at C-terminus)
Concentration: 0.25 mg/ml (determined by Absorbance at 280nm)
Appearance: Liquid protein
Physical form description: Liquid
Reconstitution Instructions:
Amino acid sequence: 346 aa (16-355 aa)
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans
