Product Description
APAF1 polyclonal Antibody | BS60866 | Bioworld
Host: Rabbit
Reactivity: Human
Application: WB IHC
Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200
Background: The mammalian homologs of the Ced-4 proteins, Apaf-1 (Ced-4), Nod1 (CARD4), and Nod2 contain a caspase recruitment domain (CARD) and a putative nucleotide binding domain, signified by a consensus Walker's A box (P-loop) and B box (Mg2+-binding site) . Nod1 contains a putative regulatory domain and multiple leucine-rich repeats. Nod1 is a member of a growing family of intracellular proteins which share structural homology to the apoptosis regulator Apaf-1. Nod1 associates with the CARD-containing kinase RICK and activates NFκB. The self-association of Nod1 mediates proximity of RICK and the interaction of RICK with IKKγ. In addition, Nod-1 binds to multiple caspases with long prodomains, but specifically activates caspase-9 and promotes caspase-9-induced apoptosis. Nod2 is composed of two N-terminal CARDs, a nucleotide-binding domain, and multiple C-terminal leucine-rich repeats. The expression of Nod2 is highly restricted to monocytes, and activates NFκB in response to bacterial lipopoly-saccharides.
Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: APAF1 polyclonal Antibody detects endogenous levels of APAF1 protein.
Molecular Weight: ~ 135 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Names: Apoptotic protease-activating factor 1; APAF-1; APAF1; KIAA0413
Immunogen: A synthetic peptide corresponding to residues in Human APAF1.
Conjugate: Unconjugated
Modification: Unmodification
Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Pathway: