Product Description
PR polyclonal Antibody | GCP29 | Bioworld
Host: Rabbit
Reactivity: Human
Application: WB IHC
Application Range: WB: 1:2000~1:5000 IHC: 1:50~1:200
Background: Human progesterone receptor (PR) is expressed as two forms: the full length PR-B and the short form PR-A. PR-A lacks the first 164 amino acid residues of PR-B. Both PR-A and PR-B are ligand activated, but differ in their relative ability to activate target gene transcription. The activity of PR is regulated by phosphorylation; at least seven serine residues are phosphorylated in its amino-terminal domain. Three sites (Ser81, Ser102, and Ser162) are unique to full length PR-B, while other sites (Ser190, Ser294, Ser345, and Ser400) are shared by both isoforms. Phosphorylation of PR-B at Ser190 (equivalent to Ser26 of PR-A) is catalyzed by CDK2. Mutation of Ser190 results in decreased activity of PR, suggesting that the phosphorylation at Ser190 may be critical to its biological function.
Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: PR polyclonal Antibody detects endogenous levels of PR protein.
Molecular Weight: ~ 125 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Names: Progesterone receptor; PR; Nuclear receptor subfamily 3 group C member 3; PGR; NR3C3
Immunogen: Synthetic peptide, corresponding to Human PR.
Conjugate: Unconjugated
Modification: Unmodification
Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Pathway: N/A