Product Description
BIK/ NBK (phospho-T33) polyclonal Antibody | BS4215 | Bioworld
Host: Rabbit
Reactivity: Human
Application: WB IHC
Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200
Background: The Bcl-2 gene was isolated at the chromosomal breakpoint of t-bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. A protein designated Bax p21 (i.e., Bcl-associated X protein) has extensive amino acid homology with Bcl-2 and both homodimerizes and heterodimerizes with Bcl-2. Overexpression of Bax accelerates apoptotic death. Natural born killer (NBK), also known as Bik, is a protein that is functionally related to Bax, although the two proteins share very little sequence homology. NBK does not contain the conserved Bcl-2 homology domains (BH domains) characteristic of the Bcl-2 family. It does however, share nine amino acids with Bax in a region designated BH3, which may be the critical determinant for the protein’s death-promoting activities. Phosphorylation of NBK at Ser35 and Thr33 is required for eliciting efficient apoptotic activity.
Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: p-NBK (T33) polyclonal Antibody detects endogenous levels of NBK protein only when phosphorylated at Thr33.
Molecular Weight: ~ 23 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Names: Apoptosis inducer NBK; BBC1; Bcl-2-interacting killer; BCL2 interacting killer; bhikhari; BIK; Bik-like killer protein; BIK; BIP 1; BIP1; BP 4; BP4; cb 60; NBK;
Immunogen: Synthetic phosphopeptide derived from human BIK/ NBK around the phosphorylation site of Threonine 33.
Conjugate: N/A
Modification: N/A
Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Pathway: N/A