Product Description
ENDOG polyclonal Antibody | BS70531 | Bioworld
Host: Rabbit
Reactivity: Human,Mouse,Rat
Application: WB IHC
Application Range: WB 1:200 - 1:500 IHC 1:50 - 1:100
Background: Endonuclease G (ENDOG), a nuclear encoded protein, localizes to the mitochondria (1) . This sugar-nonspecific nuclease, responsible for major mitochondrial nuclease activity, preferentially cleaves single-stranded DNA (ssDNA) . Synthesized as a propeptide with an amino-terminal presequence that targets the nuclease to mitochondria, ENDOG translocates to neuclei on apoptotic stimulation and act as a nuclease without sequence specificity. Both exonucleases and DNase I stimulate the ability of ENDOG to generate double-stranded DNA cleavage products at physiological ionic strengths, suggesting that these activities work in concert with ENDOG in apoptotic cells to ensure efficient DNA breakdown.
Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: ENDOG polyclonal Antibody detects endogenous levels of ENDOG protein.
Molecular Weight: ~ 33 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Names: ENDOG; Endonuclease G mitochondrial;
Immunogen: Recombinant protein of human ENDOG.
Conjugate: Unconjugated
Modification: Unmodification
Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Pathway: