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Product Description
MSK1 (phospho-Thr581) polyclonal Antibody | AP0095 | Bioworld
Host: Rabbit
Reactivity: Human,Mouse,Rat
Application: WB
Application Range: WB: 1:500~1:2000
Background: MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress. Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term) . Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain.
Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: p-MSK1 (Thr581) polyclonal Antibody detects endogenous levels of MSK1 protein when phosphorylated at Thr581.
Molecular Weight: ~ 90 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Names: Ribosomal protein S6 kinase alpha-5; S6K-alpha-5; 90 kDa ribosomal protein S6 kinase 5; Nuclear mitogen- and stress-activated protein kinase 1; RSK-like protein kinase; RSKL; RPS6KA5; MSK1
Immunogen: Synthetic phosphopeptide derived from human MSK1 around the phosphorylation site of Threonine 581.
Conjugate: Unconjugated
Modification: Phosphorylation
Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE) .
Pathway:
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