Product Description
Copper assay kit (3,5-DiBr-PAESA)
[ Product Description ]
Inside human body, approximately 95% of copper in the plasma is attached to alpha-2- globulin, ceruloplasmin and some enzymes with ferroxidase activity. The main role of protein containing copper is oxidation-reduction function. Copper deficiency is known to be associated with some diseases such as heart disease, osteoporosis, osteoarthritis, Menkes syndrome and Wilson's disease.
It has been also reported that copper deficiency may cause reduction of anti-oxidative function in vivo. On the other hand, the excess copper is toxic. This kit is a simple colorimetric assay which can detect copper precisely in biological fluids in a short period of time (about 10 minutes).
[ Assay principle ]
In this assay system, copper is dissociated from ceruloplasmin and other copper containing proteins, by denaturing agent in acidic condition.
Free and protein-associated copper will form a copper -3-5 DiBr-PAESA complex, which can be detected by absorbance at 580 nm.
[ Performance ]
1. Sensitivity: 2. Accuracy: 3. Intra assay variation: 4. Assay range: |
O.D. at 200 µg/dL standard will be 0.03 - 0.1 ±10% of known concentration C.V. is less than 5% 3 - 400 µg/dL |
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[ References: Assay examples ] | ||||||||
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