Product Description
15P-1 cell line | EP-CL-0606 | Elabscience
Background: The 15P-1 cell line was established from testicular cells of transgenic mice that express the large T protein of polyoma virus (PyLT) in the seminiferous epithelium.
Species: Mus musculus, transgenic, mous
Age: Male
Tissue: Testis
Morphology: Epithelial
Growth Properties: Adherent
Organism: Mus musculus, transgenic, mouse, transgenic
Species Cell: Mouse
application: N/A
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:5
Renewal: Every 2 to 3 days