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![293 [HEK-293] Cell Line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/904687/1196888/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__39675.1664298181.png?c=2 "293 [HEK-293] Cell Line")
![293 [HEK-293] Cell Line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/904687/1196888/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__39675.1664298181.png?c=2 "293 [HEK-293] Cell Line")
Product Description
293 [HEK-293] Cell Line | EP-CL-0001 | Elabscience
Background: Although an earlier report suggested that the cells contained Adenovirus 5 DNA from both the right and left ends of the viral genome [RF32764], it is now clear that only left end sequences are present.The cells express an unusual cell surface receptor for vitronectin composed of the integrin beta-1 subunit and the vitronectin receptor alpha-v subunit.The Ad5 insert was cloned and sequenced, and it was determined that a colinear seqment from nts 1 to 4344 is integrated into chromosome 19(19q13.2).
Species: Homo sapiens, Human
Age: Fetus
Tissue: Embryonic kidney
Morphology: Epithelial
Growth Properties: Adherent
Organism: Homo sapiens, Human
Species Cell: Human
application: Efficacy testing. Transfection host. Viruscide testing
Doubling Time: ~24-30 hours
Biosafety: 2
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:4-1:8
Renewal: Every 2 to 3 days
![293 [HEK-293] Cell Line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/904687/1196888/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__39675.1664298181.png?c=2 "293 [HEK-293] Cell Line")
![293[HEK-293] Cells Complete Medium](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/450490/653572/elabscience__13538.1643193164__44410.1643194172__40759.1648028212.png?c=2 "293[HEK-293] Cells Complete Medium")
![293T [HEK-293T] Cell Line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/904691/1196892/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__83111.1664298182.png?c=2 "293T [HEK-293T] Cell Line")
