Product Description
3T3-L1 Cell Line | EP-CL-0006 | Elabscience
Background: The cells undergo a pre-adipose to adipose like conversion as they progress from a rapidly dividing to a confluent and contact inhibited state. A high serum content in the medium enhances fat accumulation. Tested and found negative for ectromelia virus(mousepox).
Species: Mus musculus, Mouse
Age: Embryo
Tissue: Embryo
Morphology: Fibroblast
Growth Properties: Adherent
Organism: Mus musculus, Mouse
Species Cell: Mouse
application: Transfection host.
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:3-1:5
Renewal: 2 to 3 times per week