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![BHK-21 [C-13] Cell Line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/904711/1196912/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__66469.1664298187.png?c=2 "BHK-21 [C-13] Cell Line")
![BHK-21 [C-13] Cell Line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/904711/1196912/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__66469.1664298187.png?c=2 "BHK-21 [C-13] Cell Line")
Product Description
BHK-21 [C-13] Cell Line | EP-CL-0034 | Elabscience
Background: This cell line is reverse transcriptase negative.
Species: Mesocricetus auRatus, Hamster,
Age: 1 day
Tissue: Kidney
Morphology: Fibroblast
Growth Properties: Adherent
Organism: Mesocricetus auRatus, Hamster, Syrian golden
Species Cell: Hamster
application: The World Organization for Animal Health(OIE) uses these cells for routine diagnosis of rabies. This cell line has been used as a host for transformation with expression vectors containing selectable and amplifiable marker DNAs.
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days
![BHK-21 [C-13] Cell Line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/904711/1196912/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__66469.1664298187.png?c=2 "BHK-21 [C-13] Cell Line")
![BHK-21 [C-13] Cells Complete Medium](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/450515/653597/elabscience__13538.1643193164__44410.1643194172__42223.1648028215.png?c=2 "BHK-21 [C-13] Cells Complete Medium")
![C127 [C-127] Cell Line](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/904719/1196920/elabscience__13538.1643193164__44410.1643194172__90041.1647951641__22463.1664298189.png?c=2 "C127 [C-127] Cell Line")
