Product Description
Ca Ski Cell Line | EP-CL-0048 | Elabscience
Background: The cells are reported to contain an integrated human papillomavirus type 16 genome(HPV-16, about 600 copies per cell) as well as sequences related to HPV-18. ATCC has confirmed this cell line is positive for the presence of human papilloma virus(HPV) viral sequences via PCR.
Species: Homo sapiens, Human
Age: 40 years adult
Tissue: Cervix, derived from metastatic site: small intestine
Morphology: Epithelial
Growth Properties: Adherent
Organism: Homo sapiens, Human
Species Cell: Human
application: N/A
Doubling Time: N/A
Biosafety: 2
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days