Product Description
CAL 27 Cell Line | EP-CL-0265 | Elabscience
Background: CAL 27 cells are epithelial, polygonal with a highly granular cytoplasm. Immunocytochemical studies show strong positive staining with anti keratin antibodies. The cells do not grow well in semi-solid medium. Marked inhibition of Thymidine incorporation was observed in the presence of VP16(etoposide), CCNU(1-[2-chloroethyl]-3-cyclohexyl-1-nitrosourea), VM26(teniposide), ADM(adriamycin), CPA(cyclophosphamide), and MTX(Methotrexate). CAL 27 cells were resistant to treatment with VDS(vindesine sulfate), CDP(cis-platinum) or ACTD(actinomycin D). A culture submitted to the ATCC in December 1993 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.
Species: Homo sapiens, Human
Age: 56 years
Tissue: Tongue
Morphology: Epithelial
Growth Properties: Adherent
Organism: Homo sapiens, Human
Species Cell: Human
application: N/A
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days