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Product Description
CHO-K1 Cell Line | EP-CL-0062 | Elabscience
Background: The cells require Proline in the medium for growth.The CHO-K1 cell line was derived as a subclone from the parental CHO cell line initiated from a biopsy of an ovary of an adult Chinese hamster by T. T. Puck in 1957. The cells require Proline in the medium for growth.
Species: Cricetulus griseus, Hamster, C
Age: N/A
Tissue: Ovary
Morphology: Epithelial
Growth Properties: Adherent
Organism: Cricetulus griseus, Hamster, Chinese
Species Cell: Hamster
application: Transfection host.
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days
