Product Description
COLO 205 Cell Line | EP-CL-0066 | Elabscience
Background: The line was derived from tissue from the same patient as COLO 201. The cells are CSAp negative(CSAp-). The cells are positive for keratin by immunoperoxidase staining. COLO 205 cells express a 36000 dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen.
Species: Homo sapiens, Human
Age: 70 years
Tissue: Colon, derived from metastatic site: ascites
Morphology: Epithelial
Growth Properties: Mixed, Adherent And Suspension
Organism: Homo sapiens, Human
Species Cell: Human
application: Transfection host.
Doubling Time: ~25 hours
Biosafety: 1
Subculturing: Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days