Product Description
CTLA4 Ig-24 cell line | EP-CL-0284 | Elabscience
Background: CTLA4 Ig-24 is a spontaneously immortalized cell line from an adult female Chinese hamster (Cricetulus griseus). Transfected with human CTLA-4 gene (extracellular domain fused to the hinge, CH2 and CH3 regions of IgC.gamma.1 domain), CTLA4 Ig-24 express a fusion protein with a domine of CTLA4Ig.
Species: Cricetulus griseus, Hamster, C
Age: female
Tissue: Ovary
Morphology: Epithelial
Growth Properties: Adherent
Organism: Cricetulus griseus, Hamster, Chinese
Species Cell: Hamster
application: N/A
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1: 3-1: 4
Renewal: 2-3 times per week