Product Description
DH82 Cell Line | EP-CL-0073 | Elabscience
Background: The DH82 cell line has a macrophAge-like morphology, and is able to phagocytize latex particles.They are positive for Fc gamma receptors and negative for Fc mu and C3b receptors.The cells do not produce IL-1.
Species: Canis familiaris
Age: 10 years
Tissue: MacrophAge
Morphology: MacrophAge
Growth Properties: Adherent
Organism: Canis familiaris
Species Cell: Dog
application: N/A
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days