Product Description
Hey Cell Line | EP-CL-0671 | Elabscience
Background: N/A
Species: Homo sapiens, Human
Age: Age unspecified
Tissue: Ovary
Morphology: Epithelial
Growth Properties: Adherent
Organism: Homo sapiens, Human
Species Cell: Human
application: N/A
Doubling Time: 30 hours (PubMed=4016745); 20.53 hours (https://www.synapse.org/#!Synapse:syn2347014).
Biosafety: N/A
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:3-1:5
Renewal: N/A