Product Description
hFOB 1.19 Cell Line | EP-CL-0353 | Elabscience
Background: Cells grown at a permissive temperature of 33.5℃ exhibit rapid cell division(Doubling time of 36 hrs), whereas little cell division occurs at a restrictive temperature of 39.5℃(Doubling time of 96 hrs).The cells have the ability to differentiate into mature osteoblasts expressing the normal osteoblast phenotype. At the restrictive temperatures, cell division is slowed, differentiation increases, and a more mature osteoblast phenotype is produced.
Species: Homo sapiens, Human
Age: Fetus
Tissue: Bone
Morphology: Osteoblast
Growth Properties: Adherent
Organism: Homo sapiens, Human
Species Cell: Human
application: The cells provide a homogenous, rapidly proliferating model system for studying normal human osteoblast differentiation, osteoblast physiology, and hormonal, growth factor, and other cytokine effects on osteoblast function and differentiation.
Doubling Time: N/A
Biosafety: 2
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days