Product Description
HL-60 Cell Line | EP-CL-0110 | Elabscience
Background: HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, Hypoxanthine, phorbol myristic acid(PMA, TPA), dimethylsulfoxide(DMSO, 1% to 1.5%), actinomycin D, and retinoic acid.The cells exhibit phagocytic activity and responsiveness to chemotactic stimuli.The line is positive for myc oncogene expression.
Species: Homo sapiens, Human
Age: 36 years
Tissue: Peripheral blood
Morphology: Myeloblast
Growth Properties: Suspension
Organism: Homo sapiens, Human
Species Cell: Human
application: Transfection.
Doubling Time: N/A
Biosafety: 1
Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 1×10^5 and 1×10^6 viable cells/ml.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days