Product Description
HT22 Cell Line | EP-CL-0697 | Elabscience
Background: HT-22 is an immortalized mouse hippocampal cell line subcloned from the HT-4 cell line (1). The parental HT-4 cell line was derived from the immortalization of mouse neuronal tissues with a temperature sensitive SV40 T-antigen (2). HT-22 is highly sensitive to glutamate and is thus frequently used as a model system to study glutamate-induced toxicity in neuronal cells.
Species: Mus musculus, mouse
Age: N/A
Tissue: Brain; hippocampus
Morphology: Epithelial
Growth Properties: Adherent
Organism: Mus musculus, mouse
Species Cell: Mouse
application: N/A
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:3 to 1:6
Renewal: N/A