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Product Description
J774A.1 Cell Line | EP-CL-0370 | Elabscience
Background: J774A. 1 cells are active in antibody dependent phagocytosis. Their growth is inhibited by dextran sulfate, PPD and LPS. They synthesize large amounts of lysozyme and exhibits minor cytolysis but predominantly antibody-dependent phagocytosis. Interleukin 1 beta(Il1b) is synthesized continuously by this line.
Species: Mus musculus, Mouse
Age: Adult
Tissue: Ascites
Morphology: MacrophAge
Growth Properties: Adherent
Organism: Mus musculus, Mouse
Species Cell: Mouse
application: Biological response. Transfection host
Doubling Time: N/A
Biosafety: 1
Subculturing: Subcultures are prepared by scraping or blowing. For a 100mm disk,remove all but 10 mL culture medium (adjust amount accordingly for otherculture vessels). Dislodge cells from the flask substrate with a cell scraperor blowing directly with the pipettes; aspirate and add appropriatealiquots of the cell suspension into new culture vessels.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days
