Product Description
KYSE-410 cell line | EP-CL-0586 | Elabscience
Background: This cell line was established from the poorly differentiated invasive esophAgeal squamous cell carcinoma from the cervical esophagus of a 51-year-old Japanese male prior to treatment (tumor invasion into the adventitia was obvious). The cells were reported to have an overexpression of heparin binding growth factor (hst-1) and cyclin D.
Species: Homo sapiens, Human
Age: male, 51 years
Tissue: EsophAgeal Squamous Cell Carcinoma
Morphology: Epithelial
Growth Properties: Adherent
Organism: Homo sapiens, Human
Species Cell: Human
application: N/A
Doubling Time: ~32-45 hours
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1: 4-1: 6
Renewal: Every 2 to 3 days