Product Description
L Wnt-3A Cell Line | EP-CL-0375 | Elabscience
Background: The Wnt-3A gene encodes a secreted glyoprotein with a variety of signaling effects. Wnt genes control many of the patterning and growth events during embryonic development. The cells secrete biologically active Wnt-3A protein. They are presently the best source for production of Wnt-3A conditioned medium. Since the conditioned medium contains other factors besides the Wnt-3A protein, it is necessary to control any experiments involving the Wnt-3A conditioned medium with control conditioned medium from the parental cell line.
Species: Mus musculus, Mouse
Age: 100 days
Tissue: Subcutaneous connective tissue, areolar and adipose
Morphology: Fibroblast
Growth Properties: Adherent
Organism: Mus musculus, Mouse
Species Cell: Mouse
application: This cell line is presently the best source for production of Wnt-3A conditioned medium.
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days