Product Description
LN229 cell line | EP-CL-0578 | Elabscience
Background: The LN-229 cell line was established in 1979 from cells taken from a patient with right frontal parieto-occipital glioblastoma.
Species: Homo sapiens, human
Age: Female,60 years
Tissue: Brain/right frontal parieto-occipital cortex
Morphology: Epithelial
Growth Properties: Adherent
Organism: Homo sapiens, human
Species Cell: Human
application: N/A
Doubling Time: ~31 hours
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:5
Renewal: Every 2 to 3 days