Product Description
MADB106 cell line | EP-CL-0148 | Elabscience
Background: MADB106 was isolated and established from adenocarcinoma of female Fischer 344, Rattus norvegicus.
Species: Rattus norvegicus, Rat
Age: N/A
Tissue: N/A
Morphology: Epithelial
Growth Properties: Adherent
Organism: Rattus norvegicus, Rat
Species Cell: Rat
application: N/A
Doubling Time: N/A
Biosafety: N/A
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1: 2-1: 4
Renewal: Every 2 to 3 days