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Product Description
MC3T3-E1 Subclone 14 Cell Line | EP-CL-0378 | Elabscience
Background: The subclones were selected for high or low osteoblast differentiation and mineralization after growth in medium containing ascorbic acid. The MC3T3-E1 Subclone 4 and the MC3T3 Subclone 14 lines exhibit high levels of osteoblast differentiation after growth in ascorbic acid and 3 to 4mM inorganic phosphate.
Species: Mus musculus, Mouse
Age: Newborn
Tissue: Bone/calvaria
Morphology: Fibroblast
Growth Properties: Adherent
Organism: Mus musculus, Mouse
Species Cell: Mouse
application: These cell lines are good models for studying in vitro osteoblast differentiation, particularly ECM signaling. They have behavior similar to primary calvarial osteoblasts.
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days
