Product Description
MIA PaCa-2 cell line | EP-CL-0627 | Elabscience
Background: The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male.
Species: Homo sapiens, human
Age: Male,65 years
Tissue: Pancreas
Morphology: Attached epithelial with floating rounded cells
Growth Properties: Adherent, single cells and loosely attached clusters
Organism: Homo sapiens, human
Species Cell: Human
application: N/A
Doubling Time: ~40
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:5
Renewal: Every 2 to 3 days