Product Description
NCI-H23 cell line | EP-CL-0397 | Elabscience
Background: This line was established from lung cancer tissue obtained from a 59-year-old black male lung adenocarcinoma patient prior to therapy. The cells carry the mutation of K-ras 12 and a mutation in the codon 246 (ATC →ATG, isoleucine -> methionine) of the p53 gene. The cells express C-myc, L-myc, v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras and N-ras RNAs. The cell line has a heterogeneous mRNA expression for PDGF A and B chain, transforming growth factor alpha and beta and the epidermal growth factor receptor (EGFR). NCI-H23 exhibits a 20-fold higher level of c-myc DNA amplification without detectable c-myc RNA amplification. The cells stain positive for keratins 5+8 and 18 and vimentin but negative for neurofilament. The cells are L-dopa decarboxylase-negative. The cells can form colonies in soft agarose with an efficiency of 9.7%.
Species: Homo sapiens, Human
Age: male, 51 years
Tissue: Lung
Morphology: Epithelial
Growth Properties: Adherent
Organism: Homo sapiens, Human
Species Cell: Human
application: N/A
Doubling Time: ~38 hours
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1: 3-1: 4
Renewal: Every 2 to 3 days