Product Description
NCI-H838 cell line | EP-CL-0406 | Elabscience
Background: The line was isolated in 1984 from the lymph node metastases of a 59-year-old white male smoker with non-small cell lung cancer.
Species: Homo sapiens, Human
Age: male, 59 years
Tissue: Lung; From The Lymph Node Metastases
Morphology: Epithelial
Growth Properties: Adherent
Organism: Homo sapiens, Human
Species Cell: Human
application: N/A
Doubling Time: ~55 hours
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1: 3-1: 4
Renewal: 2-3 times per week