Product Description
OK Cell Line | EP-CL-0175 | Elabscience
Background: N/A
Species: Didelphis marsupialis virginia
Age: Adult
Tissue: Kidney, cortex/proximal tubule
Morphology: Epithelial
Growth Properties: Adherent
Organism: Didelphis marsupialis virginiana, Opossum
Species Cell: Other
application: This cell line is a suitable transfection host. This line was originally developed for use as a source of X chromosomes in a study of X inactivation, and was subsequently found to be an excellent cell culture model for the kidney proximal tubule epitheliu
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days