Product Description
P3/NSI/1-Ag4-1 [NS-1] cell line | EP-CL-0409 | Elabscience
Background: This cell line is a subclone of P3X27. The cells synthesize Kappa light chains but not secret. The cells are resistant to 8-azaguanine (20 μg/mL) but sensitive to HAT. Due to a deficiency in 3-ketosteroid reductase activity, the cells have been reported to be cholesterol auxotrophs. This cell line was tested for being not infected by ectromelia virus (mousepox).
Species: Mus musculus, Mouse
Age: N/A
Tissue: B Lymphocyte; Phlogocyte; Myeloma
Morphology: Lymphoblast
Growth Properties: Suspension
Organism: Mus musculus, Mouse
Species Cell: Mouse
application: N/A
Doubling Time: ~24 hours
Biosafety: 1
Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
Ratio: 1: 2-1: 4
Renewal: Every 2 to 3 days