Product Description
PC-12 Cell Line (Low differentiation) | EP-CL-0480 | Elabscience
Background: The cells do not synthesize epinephrine. This adherent variant(PC-12 Adh) has been adapted to Corning CellBIND® flasks to improve cell attachment.
Species: Rattus norvegicus, Rat
Age: N/A
Tissue: Adrenal gland
Morphology: Polygonal
Growth Properties: Adherent
Organism: Rattus norvegicus, Rat
Species Cell: Rat
application: N/A
Doubling Time: N/A
Biosafety: 1
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days