Product Description
R 1610 cell line | EP-CL-0420 | Elabscience
Background: This cell line is a clonal derivative of V79 cells that had been treated with ethylmethanesulfonate. The cells are deficient in galactokinase and are resistant to 2-deoxygalactose and 8-azaguanine.
Species: Cricetulus griseus, Hamster
Age: male
Tissue: Lung
Morphology: Fibroblast-Like
Growth Properties: Adherent
Organism: Cricetulus griseus, Hamster
Species Cell: Hamster
application: N/A
Doubling Time: N/A
Biosafety: N/A
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1: 2-1: 4
Renewal: 2-3 times per week