Product Description
Sp2/0-Ag14 cell line | EP-CL-0445 | Elabscience
Background: This cell line is a non-immunoglobulin-secreting line created by fusing a BALB/c mouse spleen cell with the mouse myeloma P3X63Ag8. The cells are resistant to 8-azaguanine (20 μg/mL) but sensitive to HAT. This cell line was tested for being not infected by ectromelia virus (mousepox).
Species: Mus musculus, Mouse
Age: N/A
Tissue: Spleen
Morphology: Lymphoblast
Growth Properties: Suspension
Organism: Mus musculus, Mouse
Species Cell: Mouse
application: N/A
Doubling Time: N/A
Biosafety: 1
Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
Ratio: 1: 2-1: 4
Renewal: Replace half of the media every 2 to 3 days