Product Description
SV40 MES 13 Cell Line | EP-CL-0470 | Elabscience
Background: The cells form colonies in soft agar, and are positive for SV40 large T antigen.The cells display prominent cytoskeletal staining for actin, and have abundant parallel fibrils in the cytoplasm. They are reported to contract in the presence of 1×10^-6 M angiotensin Ⅱ. The cells form colonies in soft agar, and are positive for SV40 large T antigen.
Species: Mus musculus, Mouse
Age: 7 to 10 weeks
Tissue: Kidney/glomerulus
Morphology: Myofibroblast-like
Growth Properties: Adherent
Organism: Mus musculus, Mouse
Species Cell: Mouse
application: N/A
Doubling Time: N/A
Biosafety: 2
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2-1:4
Renewal: Every 2 to 3 days