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Product Description
NA/NE (Noradrenaline/Norepinephrine) ELISA Kit | E-EL-0047 | Elabscience
Type: Competitive
Assay Time: 2.5h
Sensitivity: 0.19ng/mL
Detection Range: 0.31~20ng/mL
UniProt ID: N/A
Target Name: NA/NE
Target Synonym: Norepinephrine, NE, noradrenaline, NA, NAd, norad, 4, 5-β-trihydroxyphenethylamine
Test Principale: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with NA/NE. During the reaction, NA/NE in samples or Standard competes with a fixed amount of NA/NE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to NA/NE. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of NA/NE in the samples is then determined by comparing the OD of the samples to the standard curve.
Specifivity: This kit recognizes NA/NE in samples. No significant cross-reactivity or interference between NA/NE and analogues was observed.
Research Areas: Neuroscience, Cardiovascular
Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
