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Product Description
Mouse NFKB-p65 (Nuclear Factor Kappa B p65) ELISA Kit | E-EL-M0838 | Elabscience
Type: Sandwich
Assay Time: 3.5h
Sensitivity: 0.19ng/mL
Detection Range: 0.31~20ng/mL
UniProt ID: Q04207
Target Name: NFKB-p65
Target Synonym: NFKB3, p65, RelA
Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NFKB-p65. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NFKB-p65 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NFKB-p65, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Mouse NFKB-p65. You can calculate the concentration of Mouse NFKB-p65 in the samples by comparing the OD of the samples to the standard curve.
Specifivity: This kit recognizes Mouse NFKB-p65 in samples. No significant cross-reactivity or interference between Mouse NFKB-p65 and analogues was observed.
Research Areas: Cancer, Cell Biology, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism, Microbiology, Neuroscience, Signal transduction
Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
