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Product Description
QuicKey Pro Sheep T (Testosterone)ELISA Kit | E-OSEL-S0003 | Elabscience
Type: Competitive
Assay Time: 1.5h
Sensitivity: 0.25ng/mL
Detection Range: 0.63-40ng/mL
UniProt ID: N/A
Target Name: T
Target Synonym: Testosterone，T
Test Principale: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Sheep T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Sheep T are added to the micro ELISA plate wells. Sheep T in samples (or standards) competes with a fixed amount of Sheep T on the solid phase supporter for sites on the HRP linked detection antibody specific to Sheep T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Sheep T in the samples is then determined by comparing the OD of the samples to the standard curve.
Specifivity: This kit recognizes Sheep T in samples. No significant cross-reactivity or interference between Sheep T and analogues was observed.
Research Areas: Signal Transduction, Signal Transduction, Epigenetics and Nuclear Signaling, Developmental Biology
Storage: An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
