Product Description
Cattle BSA (Cattle Serum Albumin) ELISA Kit | ELK8568 | ELK Biotechnology
Alternative Names: Cattle Albumin; Fraction V
Species: Cattle
Assay Type: Competitive Inhibition
Sensitivity: 161.2 ng/mL
Standard: 30000 ng/mL
Detection Range: 468.75-30000 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 2h
Research Area: Immune molecule
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle Serum Albumin (Cattle BSA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle Serum Albumin (Cattle BSA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle Serum Albumin (Cattle BSA) in the samples is then determined by comparing the OD of the samples to the standard curve.