Product Description
Cattle CTSD (Cathepsin D) ELISA Kit | ELK7942 | ELK Biotechnology
Alternative Names: CPSD; CLN10; Lysosomal Aspartyl Protease; Ceroid-Lipofuscinosis, Neuronal 10
Species: Cattle
Assay Type: Competitive Inhibition
Sensitivity: 0.88 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 2h
Research Area: Tumor immunity
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cathepsin D (CTSD) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cathepsin D (CTSD) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cathepsin D (CTSD) in the samples is then determined by comparing the OD of the samples to the standard curve.