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Product Description
Chicken ADP (Adiponectin) ELISA Kit | ELK2987 | ELK Biotechnology
Alternative Names: GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1
Species: Chicken
Assay Type: Sandwich
Sensitivity: 57 pg/mL
Standard: 8000 pg/mL
Detection Range: 125-8000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 3.5h
Research Area: Metabolic pathway;Infection immunity;Cardiovascular biology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adiponectin (ADP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Adiponectin (ADP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adiponectin (ADP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adiponectin (ADP) in the samples is then determined by comparing the OD of the samples to the standard curve.
