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Product Description
General 3, 4-dihydroxyphenyl acetic acid (DOPAC) Elisa Kit. | ELK8377 | ELK Biotechnology
Alternative Names: DOPAC
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 0.59 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 2h
Research Area: Metabolic pathway;Bone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DOPAC. (3, 4-dihydroxyphenyl acetic acid ) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DOPAC. (3, 4-dihydroxyphenyl acetic acid ) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DOPAC. (3, 4-dihydroxyphenyl acetic acid ) in the samples is then determined by comparing the OD of the samples to the standard curve.
