Product Description
General ALCAR (Acetylcarnitine) ELISA Kit | ELK8184 | ELK Biotechnology
Alternative Names: Acetyl-L-carnitine
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 25.2 ng/mL
Standard: 5000 ng/mL
Detection Range: 78.13-5000 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Metabolic pathway;Neuro science;Nutrition metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Acetylcarnitine (ALCAR) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Acetylcarnitine (ALCAR) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Acetylcarnitine (ALCAR) in the samples is then determined by comparing the OD of the samples to the standard curve.