Euro
USD
British Pound
NULL
Product Description
General Cit (Citrulline) ELISA Kit | ELK9241 | ELK Biotechnology
Alternative Names: Citrulline
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 0.51 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: serum, plasma, urine and other biological fluids
Assay Lenght: 2h
Research Area: Metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Citrulline (Cit) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Citrulline (Cit) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Citrulline (Cit) in the samples is then determined by comparing the OD of the samples to the standard curve.
