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Product Description
General DAG (Diacylglycerol) ELISA Kit | ELK8147 | ELK Biotechnology
Alternative Names: DG; Diglyceride
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 141 pg/mL
Standard: 100000 pg/mL
Detection Range: 1562.5-100000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 2h
Research Area: Signal transduction;Endocrinology;Hepatology;Gastroenterology;Nutrition metabolism;Hormone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Diacylglycerol (DAG) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Diacylglycerol (DAG) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Diacylglycerol (DAG) in the samples is then determined by comparing the OD of the samples to the standard curve.
