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Product Description
General fT4 (Free Thyroxine) ELISA Kit | ELK3119 | ELK Biotechnology
Alternative Names: FTI
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 0.48 pg/mL
Standard: 90 pg/mL
Detection Range: 1.41-90 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 2h
Research Area: Signal transduction
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Free Thyroxine (fT4) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Free Thyroxine (fT4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Free Thyroxine (fT4) in the samples is then determined by comparing the OD of the samples to the standard curve.
