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Product Description
General Lipoteichoic Acids (LTA) ELISA Kit | ELK8790 | ELK Biotechnology
Alternative Names: LTA
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 0.13 ng/mL
Standard: 20 ng/mL
Detection Range: 0.31-20 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Enzyme & Kinase
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LTA (Lipoteichoic Acids) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to LTA (Lipoteichoic Acids) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LTA (Lipoteichoic Acids) in the samples is then determined by comparing the OD of the samples to the standard curve.
